姓名：李寧求
職稱：研究員
學(xué)歷、學(xué)位：研究生、博士
聯(lián)系電話：13533755943
電子郵件：liningq@126.com
個(gè)人簡(jiǎn)介：
李寧求，男，博士，研究員，博士生導(dǎo)師，中國水產(chǎn)科學(xué)研究院水產(chǎn)疫苗創(chuàng)制與免疫防控創(chuàng)新團(tuán)隊(duì)首席，農(nóng)業(yè)農(nóng)村部漁用藥物創(chuàng)制重點(diǎn)實(shí)驗(yàn)室副主任。長(zhǎng)期從事鱖鱸鱧等特色魚類疫苗創(chuàng)制與免疫防控、疫病區(qū)域化控制、無病毒苗種繁育與抗病毒新品種選育等，先后主持和參與了國家自然科學(xué)基金、國家重點(diǎn)研發(fā)計(jì)劃、國家863計(jì)劃、國家科技支撐計(jì)劃（攻關(guān)計(jì)劃）、公益性農(nóng)業(yè)行業(yè)專項(xiàng)等重大項(xiàng)目20余項(xiàng)。牽頭獲中國水產(chǎn)科學(xué)研究院科技獎(jiǎng)一等獎(jiǎng)等成果獎(jiǎng)勵(lì)7項(xiàng)、共發(fā)表學(xué)術(shù)論文180余篇（第1作者或通訊作者論文80余篇）、獲授權(quán)國家發(fā)明專利30、參編著作5部。入選中國水產(chǎn)科學(xué)研究院“領(lǐng)軍人才計(jì)劃”和“中青年科技拔尖人才”等。
研究方向：
1.病原致病與宿主免疫機(jī)制
2.漁用疫苗創(chuàng)制與免疫防控
3.疫病區(qū)域化控制技術(shù)
4.無病毒苗種繁育與抗病毒新品種選育
社會(huì)兼職：
中國水產(chǎn)科學(xué)研究院學(xué)術(shù)委員會(huì)常務(wù)委員、水產(chǎn)病害防治學(xué)科專業(yè)委員會(huì)副主任委員
第一屆獸藥評(píng)審咨詢專家?guī)焐镏破方M專家
廣東水產(chǎn)學(xué)會(huì)水生動(dòng)物衛(wèi)生與免疫專業(yè)委員會(huì)副主任委員
廣東水產(chǎn)學(xué)會(huì)青年委員會(huì)副主任委員
高校校外導(dǎo)師兼職情況：
上海海洋大學(xué)博士生導(dǎo)師；中國農(nóng)業(yè)科學(xué)院碩士生導(dǎo)師；河南師范大學(xué)、廣東海洋大學(xué)、仲愷農(nóng)業(yè)工程學(xué)院、長(zhǎng)江大學(xué)等碩士生導(dǎo)師。
獲獎(jiǎng)情況：
1. 鱖鱸鱧病毒病全鏈條精準(zhǔn)防控關(guān)鍵技術(shù)研究與示范，中國水產(chǎn)科學(xué)研究院科技獎(jiǎng)一等獎(jiǎng)，排名第一。
2. 廣譜、高效海水魚弧菌病重組OmpK亞單位疫苗創(chuàng)制與轉(zhuǎn)基因生物安全評(píng)價(jià)，中國水產(chǎn)科學(xué)研究院科技進(jìn)步獎(jiǎng)三等獎(jiǎng)，排名第一。
3. 鱖魚免疫與生態(tài)防控技術(shù)示范與推廣，廣東省農(nóng)業(yè)技術(shù)推廣獎(jiǎng)二等獎(jiǎng)，排名第二。
4. 筍殼魚病害免疫研究及生態(tài)防控集成技術(shù)示范，嶺南動(dòng)植物科學(xué)技術(shù)獎(jiǎng)一等獎(jiǎng)，排名第八。
5. 海水養(yǎng)殖魚類哈維氏弧菌、溶藻弧菌二聯(lián)疫苗研究，中國水產(chǎn)科學(xué)研究院科技進(jìn)步獎(jiǎng)三等獎(jiǎng)，排名第三。
近幾年代表性論文及其他代表性成果：
1.Yinjie Niu, Saiya Ma, Hongru Liang, Xiaozhe Fu, Baofu Ma, Qiang Lin, Xia Luo and Ningqiu Li. The Stability and Efficency of CPB Cells Were Acclimated for Virus Proliferation. Vaccines 2024, 12, 220.
2.Xia Luo, Guoli Liao, Xiaozhe Fu, Hongru Liang, Yinjie Niu, Qiang Lin, Lihui Liu, Baofu Ma and Ningqiu Li. A Novel and Effective Therapeutic Method for Treating Aeromonas schubertii Infection in Channa maculate. Animals 2024, 14, 957.
3.Baofu Ma, Jingkang Li, Min Zhang, Xiaozhe Fu, Hongru Liang, Yinjie Niu, Qiang Lin, Xia Luo, Lihui Liu, Jianguo Su, Jin Zhou, Ningqiu Li. Tissue factor pathway inhibitors disrupt structures of rhabdovirus rana-iridovirus and inhibit viral infection in Chinese perch, Siniperca chuatsi. Fish and Shellfish Immunology 149 (2024) 109553
4.Xiaozhe Fu , Wenxian Li, Cong Liu, Xia Luo , Qiang Lin, Yinjie Niu , Hongru Liang ,Baofu Ma, Ningqiu Li. A naturaly attenuated largemouth bass ranavirus strain provided protection for Micropterus salmoides by immersion immunization. Fish and Shellfish Immunology 153 (2024) 109871
5.Pinhong Li, Xia Luo, Shaozhi Zuo, Xiaozhe Fu , Qiang Lin, Yinjie Niu, Hongru Liang, Baofu Ma and Ningqiu Li. Genome-Wide Association Study of Resistance to Largemouth Bass Ranavirus (LMBV) in Micropterus salmoides. Int. J. Mol. Sci. 2024, 25, 10036
6.Xia Luo, Ruoxuan Wang, Baofu Ma, Ouqin Chang, Xiaozhe Fu, Hongru Liang,Yinjie Niu, Qiang Lin, Lihui Liu , Aijun Lv, Ningqiu Li. Inactivated LMBV vaccine with nano?aluminum adjuvant provided immune protection in largemouth bass (Micropterus Salmoides). Aquaculture 595 (2025) 741634
7.Baofu Ma, Fangying Li, Xiaozhe Fu, Xia Luo, Qiang Lin, Hongru Liang, Yinjie Niu and Ningqiu Li. Asparagine Availability Is a Critical Limiting Factor for Infectious Spleen and Kidney Necrosis Virus Replication. Viruses 2024, 16, 1540
8.Yinjie Niu, Xinmei Yang, Hongru Liang, Xia Luo, Baofu Ma , Qiang Lin, Xiaozhe Fu and Ningqiu Li. scTRIM44 Positively Regulated Siniperca Chuatsi Rhabdovirus Through RIG-I- and MDA5-Mediated Interferon Signaling. Viruses 2024, 16,1876
9.Fangying Li, Xiaozhe Fu, Xia Luo, Qiang Lin, Hongru Liang,Yinjie Niu, Lihui Liu，Ningqiu Li. Role of asparagine biosynthesis pathway in Siniperca chuatsi rhabdovirus proliferation. Frontiers in Microbiology 2023，14:1165491
10.Yinjie Niu, Xiaozhe Fu, Qiang Lin, Hongru Liang, Xia Luo, Shaozhi Zuo, Lihui Liu and Ningqiu Li*. Epidermal growth factor receptor promotes infectiousspleen and kidney necrosis virus invasion via PI3K-Akt signaling pathway. Journal of General Virology 2023;104:001882
11.Yinjie Niu, Xiaozhe Fu, Qiang Lin, Hongru Liang, Xia Luo, Shaozhi Zuo, Lihui Liu, Ningqiu Li*. In vivo and in vitro, antiviral effects of two mixture of Chinese herbal drug active monomers against MSRV and LMBV in largemouth bass (Micropterus salmoides). Aquaculture 577 (2023) 739977
12.Xiaozhe Fu, Mingju Luo, Guo Zheng，Hongru Liang, Lihui Liu, Qiang Lin, Yinjie Niu, Xia Luo, Ningqiu Li*. Determination and Characterization of a Novel Birnavirus Associated with Massive Mortality in Largemouth Bass. Microbiology Spectrum 2022, 10(2): e01716-21
13.Xiaozhe Fu, Kejin Li, Yinjie Niu, Qiang Lin, Hongru Liang, Xia Luo, Lihui Liu, Ningqiu Li*.The mTOR SIRT3 Pathway Drives Reductive Glutamine Metabolism to Reduce Oxidative Stress Caused by ISKNV in CPB Cells. Microbiology Spectrum 2022, 10(1): e02310-21
14.Hongru Liang, Xiaozhe Fu, Qiang Lin, Lihui Liu, Zhibin Huang, Yinjie Niu, Luo Xia, Ningqiu Li*.Inhibition of grass carp reovirus with PEGylated aptamers. Aquaculture 559 (2022) 738412
15.Xiaoting Zhang , Yue Ming1, Xiaozhe Fu , Yinjie Niu , Qiang Lin , Hongru Liang , Xia Luo , Lihui Liu , Ningqiu Li*. PI3K/AKT/p53 pathway inhibits infectious spleen and kidney necrosis virus infection by regulating autophagy and immune responses. Fish and Shellfish Immunology 120 (2022) 648–657
16.Yinjie Niu , Xiaozhe Fu , Qiang Lin , Hongru Liang , Xia Luo , Shaozhi Zuo , Lihui Liu , Ningqiu Li*. The composition and antiviral activity of scTRIM59 in Mandarin fish. Fish and Shellfish Immunology 130 (2022) 86–92
17.Yinjie Niu, Xiaozhe Fu, Qiang Lin, Lihui Liu, Xia Luo, Hongru Liang, Ningqiu Li*. Gefitinib inhibits infectious spleen and kidney necrosis virus infection in vivo and vitro by blocking virus endocytosis. Aquaculture 539 (2021) 736614
18..Hongru Liang, Lixi Zhang, Xiaozhe Fu, Qiang Lin, Lihui Liu, Yinjie Niu, Xia Luo, Zhibin Huang and Ningqiu Li*. Development of a double-Antibody Sandwich ELISA for Rapid Detection of the MCP Antigen Concentration in Inactivated ISKNV Vaccines. Vaccines 2021, 9, 1264
19.Xia Luo, Yinjie Niu, Xiaozhe Fu, Qiang Lin, Hongru Liang, Lihui Liu and Ningqiu Li*. Large-Scale Microcarrier Culture of Chinese Perch Brain Cell for Viral Vaccine Production in a Stirred Bioreactor. Vaccines 2021, 9, 1003
20.Xiaozhe Fu, Yue Ming, Chen Li, Yinjie Niu, Qiang Lin, Lihui Liu, Hongru Liang, Zhibin Huang, Ningqiu Li*. Siniperca chuatsi rhabdovirus (SCRV) induces autophagy via PI3K/AktmTOR pathway in CPB cells. Fish and Shellfish Immunology 102 (2020) 381–388.
21.Xiaozhe Fu, Lixi Zhang, Lihui Liu, Qiang Lin, Hongru Liang, Yinjie Niu, Zhibin Huang, Ningqiu Li*. Identification of intron in ORF003 gene and its application for inactivation test of ISKNV. Microbial Pathogenesis 138 (2020) 103822
22.Yinjie Niu, Xiaozhe Fu, Lihui Liu, Qiang Lin, Hongru Liang, Zhibin Huang, Ningqiu Li*. Molecular characterization and function of EGFR during viral infection process in Mandarin fishSiniperca chuatsi. Fish and Shellfish Immunology 102 (2020) 211–217
23.Xixi Guo, Yang Zhou, Xiaozhe Fu, Qiang Lin, Lihui Liu, Hongru Liang, Yinjie Niu, Ningqiu Li*. Transcriptomic profiles reveal that inactivated iridovirus and rhabdovirusbivalent vaccine elicits robust adaptive immune responses against lethal challenge in marbled sleepy goby. Fish and Shellfish Immunology 98 (2020) 429–437
24.Qiang Lin, Jie Li, Xiaozhe Fu, Lihui Liu, Hongru Liang, Yinjie Niu, Chuni Huang,Zhibin Huang, Zhaolan Mo, Ningqiu Li*. Hemorrhagic gill disease of Chinese perch caused byAeromonas salmonicida subsp.salmonicidain China. Aquaculture 519 (2020) 734775
25.Qiang Lin, Xiaozhe Fu, Lihui Liu, Hongru Liang, Yinjie Niu, Yuanya Wen,,Zhibin Huang, Ningqiu Li*. Development and application of a sensitive droplet digital PCR (ddPCR) for the detection of infectious spleen and kidney necrosis virus .Aquaculture，2020，529：735697
